Knowledge Center is a resource library of publications with information about the SimCell™ System and its applications and methodologies.
Learn how the SimCell System can accelerate your bioprocess development and optimization.
CellLines
A periodic e-newsletter keeping you informed on the latest news in SimCell technology.
SimCell Tutorial Application Note
H. Brett Schreyer, Scott E. Miller and Seth Rodgers, Gen. Eng. News, Vol.27, No.17, p.44
SimCell MicroBioreactor technology is shown to be a predictive scale-down model for stirred-tank bioreactor systems. New workflow strategies are described to take advantage of the high-throughput, parallel nature of the multiplexed MicroBioreactor platform.
Predicting Bioreator Cell Culture Performance with a Sub-Milliliter MicroBioreactor System
H. Brett Schreyer, Rachel Legmann, BioProcessors Corp., Woburn, MA
A model CHO cell line producing human interferon-y is used to compare culture performance between bench bioreactors and SimCell MicroBioreactors. Specifically, cell growth, glucose metabolism, glutamine metabolism, interferon production, and interferon gylcosylation are examined and compared for both systems. Results demonstrate equivalency between the two systems, providing evidence that the SimCell MicroBioreactor scale-down model can predict performance of conventional stirred-tank bioreactors.
H. Brett Schreyer, Scott Miller, Tim Johnson, Seth Rodgers, BioProcessors Corp., Woburn, MA
Experimental results show excellent correlation between SimCell MicroBioreactor and benchtop bioreactor cell cultures. Optimum conditions indicated by the SimCell MicroBioreactor model were confirmed in 3L bioreactors, providing evidence of the scale-up predictability of the SimCell platform.
Implementation of High Throughput Systems for Media and Process Development
Soverin Karmiol, Mykle Gaynor, Robert Kenerson, John Daley, Rich Hassett, Steven Peppers, Invitrogen Corporation, Grand Island, NY
The SimCell System is implemented in a media and process development workflow to investigate 196 individual combinations of media components, feed regimens and process conditions. Interactions and effects of these factors on the growth of a CHO cell clone are studied. Results illustrate how the SimCell integrates into a media development and optimization workflow.
Learn more about the use of the SimCell System for media development and optimization at Invitrogen.
Evaluation of a Novel MicroBioreator System for Cell Culture Optimization
G. Wilson, Novo Nordisk A/S, Kobenhavn, Denmark
A statistical factorial experiment was conducted to examine the effect of pH and temperature shift on a bioproduction process to produce a therapeutic protein from CHO cells. Compared to the one year required to study 50 different combinations of pH and temperature using conventional benchtop fermenters, only one month would be required using an automated SimCell System. Specific productivity results from the SimCell MicroBioreactors correlated well with those from 5-L fermenters.
Novel MicroBioreactors as Scale-down Simulators of Large Stirred-Tank Reactors
Timothy J. Johnson, A Peter Russo, Brian O. Benoit and George J. Vella
Computational fluid dynamic models were developed to ensure that the fluidic micro environment of the SimCell MicroBioreactor is similar to stirred tanks. Shear range and shear frequency in the SimCell MicroBioreactor are compared to shake flasks and bioreactors. Results indicate that the SimCell MicroBioreactor is representative as a scale-down bioreactor model.
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